Image Description
Immunohistochemical Profile of Mastocytosis: Neoplastic mast cells express CD33, CD5, CD68, KIT/CD117, tryptase and chymase. CD117 (shown here) is highly sensitive but less specific marker for mast cells. Chymase is highly specific but not as sensitive. Mast cells also express chloroacetate esterase and are negative for myeloperoxidase.
Neoplastic mast cells show aberrant expression of CD25 and/or CD2. This is helpful in differentiating them from normal mast cells when the infiltrate is sparse or subtle. CD2 is more readily detected by flow cytometry. More recently CD30 has also been established as a novel aberrant marker for neoplastic mast cells especially in advanced systemic mastocytosis. Mast cells don't express most B- and T-cell markers and are negative for myelomonocytic markers CD14, CD15, and CD16.
Neoplastic mast cells show aberrant expression of CD25 and/or CD2. This is helpful in differentiating them from normal mast cells when the infiltrate is sparse or subtle. CD2 is more readily detected by flow cytometry. More recently CD30 has also been established as a novel aberrant marker for neoplastic mast cells especially in advanced systemic mastocytosis. Mast cells don't express most B- and T-cell markers and are negative for myelomonocytic markers CD14, CD15, and CD16.